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Hydroxychloroquine Sulfate

Reference Number: AA-04116 Created: 05/27/2020 02:47 PM Last Updated: 09/11/2020 04:08 PM

Method Conditions:

Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-75P
Dimensions: 4.6 x 75 mm
Solvents: A. DI H20 / 0.1% formic acid (v/v)
                B. Acetonitrile / 0.1% formic acid (v/v)

time (min.)    %B
0              80
1              80
3              50
4              50
5              80
8              80

Injection Volume: 1ul
Flow Rate: 1.0 / min
Detection: 254nm
Peak: 1. Hydroxychloroquine sulfate
Samples: 0.1 mg/mL Hydroxychloroquine sulfate in 50:50 acetonitrile:DI H2O

Discussion:  Hydroxychloroquine sulfate presents a few complexities to routine chromatographic analysis. The amine groups also interact with lone-silanols, causing peak tailing on regular silica columns. In typical reversed phased modes, buffers are needed in higher concentrations to aid in retention, as well as ion pair reagents. These additives can hinder coupling in mass spectrometry and also increases the likelihood of source contamination. However, use of the Cogent Diamond Hydride™ column in ANP illustrates how Hydroxychloroquine can be readily retained, with good run-to-run precision, and possess excellent peak shape without ion pairing reagents. (%RSD = 0.09, SD below 0.004.) This method demonstrates an easy HPLC method that can be quite readily transferred to LCMS.

Note: Hydroxychloroquine is an inexpensive antimalarial drug, now regarded as a safe and reasonably effective treatment for various autoimmune rheumatic diseases including systemic lupus erythematosus.

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