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Dantrolene Sodium

Reference Number: AA-04112 Created: 05/26/2020 05:11 PM Last Updated: 09/11/2020 04:09 PM

Method Conditions:

Column: Cogent Bidentate C18™, 4μm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75 mm
Injection Volume: 2μL
Flow Rate: 1.0ml / min
Detection: 225 nm
          0.1 mg/mL Dantrolene sodium in 50:50 acetonitrile: DI H2O
Peak: 1. Dantrolene sodium

Mobile Phase: 60% DI H2O / 40% acetonitrile / 0.1% formic Acid (v/v)

Discussion: Dantrolene sodium has several amine groups that can interact with lone-silanols causing problematic peak tailing when analyzed with conventional HPLC columns. By utilizing a Bidentate C18™ column with its Silica Hydride™ surface, challenging compounds like Dantrolene can be readily retained with symmetrical peak shape. 

Note: Essential to muscle contraction are ryanodine receptors that regulate the release of calcium from the sarcoplasmic reticulum of muscle cells. Dantrolene sodium is a postsynaptic muscle relaxant that lessens the “excitation-contraction” coupling response in these cells. It achieves this by inhibiting calcium binding to ryanodine receptor 1 and decreasing intracellular calcium concentrations.


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