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Dimethylolpropionic Acid

Reference Number: AA-03872 Created: 04/07/2020 09:52 AM Last Updated: 09/11/2020 04:25 PM

In this application note, we demonstrated how easy you can retain an organic acid in Aqueous Normal Phase with Silica Hydride technology. 

Method Conditions:
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Solvents: A: DI H2O/ 10 mM ammonium formate (v/v)
B: 95% Acetonitrile/ 5% DI H2O/ 10 mM ammonium formate (v/v)

Isocratic: 90%B

Injection Volume: 5μL

Flow Rate: 1.0ml / min
Detection: 215 nm

Peak: 1. Dimethylolpropionic acid 

         2. Sample diluent (Mobile Phase B) 


          3mg/mL (Diluent: Mobile Phase B)

t0: 0.94 min

Discussion: Dimethylolpropionic Acid is a unique diol molecule with both hydroxyl and carboxyl functionality. This compound presents several challenges in chromatographic analysis. Traditional reversed phase mode may not be viable due to the high hydrophobicity of this organic acid. Also, by lacking chromophores it can be difficult to detect using conventional HPLC techniques. By pairing the Cogent Diamond Hydride™ column with Aqueous Normal Phase this method has proven both detection without the use of derivatization and excellent retention of a very polar compound. The presented data illustrates how the compound can be retained with exceptional peak shape. If extended retention is desired, a longer dimension column can be employed.

Note: Dimethylolpropionic acid is often used to provide carboxylate functional group and provide good hydrophilic character for the polyurethane polymer. Dimethylolpropionic acid based dendrimers may have potent applications as delivery systems for anticancer drugs such as cisplatin and doxorubicin, as well as agents for both boron neutron capture therapy and photodynamic therapy.

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