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A cannabis metabolite in urine matrix

Reference Number: AA-03366 Created: 03/13/2019 02:23 PM Last Updated: 03/13/2019 02:42 PM

A cannabis metabolite in urine matrix
Peak: (l)-9-Carboxy-11-Nor-Delta-9- THC, m/z 345.2060 [M+H]+

Method Conditions:
Column: Cogent Phenyl Hydride 4um, 100A
Catalog No.: 69020-05P-2
Dimensions: 2.1 50mm
Solvents: A. DI H20 / 0.1% formic acid (v/v)
                B. Methanol / 0.1% formic acid (v/v)
Gradient:
time (min.)    %B
     0              10
     3              10
     7              80
     8              10

Post Time: 3 min
Injection Volume: 1ul
Flow Rate: 0.4ml / min
Detection: ESI - POS - Agilent 6210 MSD TOF mass spectrometer
t0: 0.9 min
Samples: Urine sample was loaded into an SPE cartridge II (Clean Screen Xcel, UCT Bristol, PA, USA) and eluted with 1 mL of acetonitrile, 2-propanol, formic acid (50/50/1).  After elution, the sample was dried under nitrogen gas and dissolved in 100 microL of 50% methanol / 50% DIwater / 0.1% formic acid. The solution was filtered through a 0 .45µm AQ™ nylon syringe filter (MicroSolv Tech Corp)

Discussion:
The extraction procedure using  an SPE technique allows  for successful detection of this cannabis metabolite with the Cogent Phenyl Hydride column. It is worth noting that the retention of this compound was achieved using methanol rather than acetonitrile as the organic component in the mobile phase.  The Cogent Phenyl Hydride phase provides excellent retention for analytes with rigid aromatic rings.

Note: (l)-9-Carboxy-11-Nor-Delta-9- THC  is the main  metabolite of tetrahydrocannabinol (THC), formed in the body after consumption of cannabis.  The compound stays in the body for a significant time, making it useful as a test compound for cannabis use. In the U.S., cannabis is a controlled substance at the Federal level, although two states have recently enacted laws legalizing it.

   
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