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What does "residual volume" mean when referring to autosampler vials?

Reference Number: AA-01176 Created: 01/10/2013 03:09 PM Last Updated: 09/10/2020 02:40 PM

The syringe needle of an autosampler does not go all the way to the bottom of a vial, and therefore some sample will always remain. This is referred to as the residual volume of the vial. Some vials have a tapered bottom for example, which decreases the residual volume. This can be important if the sample is valuable and of limited amount. .

However, the residual volume value is nominal. Every situation is unique because autosamplers have different depths that they travel in the vial and some have side port needles and some have bottom port.

The following diagram illustrates how residual volume will remain after the syringe needle can no longer aspirate the liquid:

Only the AQ and RSA vials have a specification for bottom height. The tolerance of this bottom height allows you to bring the needle down to the lowest point possible with out worry that other vials will have a different bottom height

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