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Organic Alcohols

Reference Number: AA-01064 Created: 11/28/2012 02:13 PM Last Updated: 09/09/2020 09:43 AM

1-Phenyl-2-propanol and 2-Phenyl-1,2-propanediol  

Organic Alcohols

                                     Peak: 1. 2-Phenyl-1,2-propanediol  2. 1-Phenyl-2-propanol

     Method Conditions

      Column: Cogent  Bidentate C18, 4µm, 1 00Å
      Catalog No.: 40018-75P
      Dimensions: 4.6 x 75 mm
      Solvents: A: DI  H20  /  0 .1% formic  Acid (v/v)
                      B: Acetonitrile/ 0.1% formic Acid (v/v)

              time (min.)     %B

                           0       10 
  2       10 
  6       50
  7       10

      Injection vol.: 5 µL 
      Flow rate: 1.0 ml/min 
      Detection: UV 220 nm
       Post time: 3 min  
      Samples: 0.1 µL/mL 1-Phenyl-2-propanol and 0.1 mg/mL 2-Phenyl-1,2- propanediol in diluent of 50/50 solvent A / solvent B.
      t00.9 min


In this method, separation is obtained between two structurally similar  organic alcohols. The method illustrates the type of retention and selectivity that can be obtained for these kinds  of compounds. Furthermore, the mobile phase solvents are simple  to prepare and LC-MS compatible. The overlay in the figure demonstrates how  the stationary phase material can be produced with reliable consistency from lot-to- lot, which is a critical feature of HPLC columns. Columns from five different material lots were  investigated and retention time RSD values of 0.5% and 0.3% were  obtained for the two peaks between the lots.

Note: These two compounds have applications as starting materials in organic syntheses. As such, the method has relevance in impurities testing of finished products to ensure  no starting materials are present.

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