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Steroids in Clinical Studies

Reference Number: AA-00798 Created: 05/17/2012 07:02 AM Last Updated: 09/04/2020 04:29 PM

       Using LC-MS to Measure  Steroids in Clinical Studies
                                                   Atmospheric-pressure chemical ionization (APCI) detection




Method Conditions
      Column: Cogent UDC Cholesterol, 4µm, 1Cogent UDC Cholesterol™, 4µm, 100Å
      Catalog No.: 69069-7.5P
      Dimensions: 4.6 x 75 mm
      Solvents: 50% DI H2O/ 50% MeOH/ 0.5% formic acid
      Injection vol.: 5 µL 
      Flow rate: 0.5 ml/min 
APCI, Single  Ion monitoring  

     Discussion:  Serum corticosterone concentrations are of clinical significance in adrenal dysfunction. Its measurement is sometimes used to diagnose apparent mineralcorticoid excess syndrome. It can also be used as a bio marker of malignancy in adrenal tumors.  Using the Cogent UDC Cholesterol column allows  the simultaneous measurement of the two main estrogen fractions, estrone and estradiol in breast tumor tissue. Highly sensitive assays for estrone and estradiol for measuring low  levels of estrogen in postmenopausal women, and monitoring estrogen levels in women receiving hormone replacement therapy can also be developed using  the method presented in this note.

     Notes:  The total ion chromatogram of a mix- ture of six steroids presented substantiates the resolving power of the Cogent UDC Cholesterol column. The peak shapes are excellent (As < 1.1 for all solutes) using  an isocratic mobile phase.

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