MicroSolv Technologies Knowledge Base

Microsolv

Browse Ask a Question
 
Tools
Categories

Steroids in Clinical Studies

Reference Number: AA-00798 Created: 05/17/2012 07:02 AM Last Updated: 03/13/2019 12:34 PM

           Using LC-MS to Measure  Steroids in Clinical Studies
                                                   Atmospheric-pressure chemical ionization (APCI) detection

                                        

Peaks:

                                                            


     
Method Conditions
      Column: Cogent UDC Cholesterol, 4µm, 10Cogent UDC Cholesterol™, 4µm, 100ÅÅ
      Catalog No.: 69069-7.5P
      Dimensions: 4.6 x 75 mm
      Solvents: 50% DI H2O/ 50% MeOH/ 0.5% formic acid
      Injection vol.: 5 µL 
      Flow rate: 0.5 ml/min 
      Detection: 
APCI, Single  Ion monitoring  
.

     Discussion:  Serum corticosterone concentrations are of clinical significance in adrenal dysfunction. Its measurement is sometimes used to diagnose apparent mineralcorticoid excess syndrome. It can also be used as a bio marker of malignancy in adrenal tumors.  Using the Cogent UDC Cholesterol column allows  the simultaneous measurement of the two main estrogen fractions, estrone and estradiol in breast tumor tissue. Highly sensitive assays for estrone and estradiol for measuring low  levels of estrogen in postmenopausal women, and monitoring estrogen levels in women receiving hormone replacement therapy can also be developed using  the method presented in this note.

     Notes:  The total ion chromatogram of a mix- ture of six steroids presented substantiates the resolving power of the Cogent UDC Cholesterol column. The peak shapes are excellent (As < 1.1 for all solutes) using  an isocratic mobile phase.

    
      © Copyright MicroSolv Technology Corporation, all rights reserved.
Attachments
No 35 Using LC-MS to Measure Steroids in Clinical Studies.pdf 0.2 Mb Download File
Info Ask a Question 
Your Email: Subject: Question: