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Assuring retention of polar compounds in an ANP gradient in positive mode LCMS.

Reference Number: AA-00303 Created: 04/02/2012 01:08 PM Last Updated: 09/02/2020 05:33 PM

When using a Cogent Diamond Hydride™ column prepare your solvents for positive ionization in LC-MS: Solvent A: 50% methanol/50% DI water + 0.1% formic acid and Solvent B: 90% acetonitrile/10% DI water/0.1% formic acid.

ANP gradients need to start between 100% - 90% of Solvent B (to retain polar compound) and then reduce the percent of Solvent B even down to 20% solvent B (when 50% methanol/50% water is used as Solvent A).

One important advantage to note about the Cogent Diamond Hydride columns is that they equilibrate between gradient runs very fast (5 minutes) compared to HILIC columns which can be 20-30 minutes.

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