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Working with plasma samples and Cogent TYPE-C HPLC columns

Reference Number: AA-00298 Created: 04/02/2012 12:47 PM Last Updated: 09/02/2020 05:31 PM

When working with plasma extracts, we recommend using 50% methanol/50% DI water for washing between injections. Flow rates of 0.5ml/min for 20-30 minutes for a 4.6mm x 250mm column should be sufficient.

When working with LC-MS and biological samples we recommend using 50% methanol/50% Di water as the A solvent. It helps clean the column between runs when biological samples are used. However, for some samples, especially those with a significant amount of lipids, an A solvent of 50:50 water/IPA is recommended. 

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